Axichrom 50 to 200 mm chromatography columns cytiva. It allows users to speed up process scaleup and cleaning between product. This procedure describes packing of capto s impact in axichrom columns using the custom mode feature of intelligent packing. Labor, medium, and water costs are all reasons to ensure the column is optimally packed the first time. Axichrom columns are designed to minimize resin loss.
Customized columns for when the standard offering does not fit your needs. How to pack axichrom columns using aktaavant 150 system duration. An exact amount of resin can be automatically packed without an external pump. They can both be used with traditional and highflow media families, such as capto and mabselect. Unsubscribe from cytiva formerly ge healthcare life sciences. Large axichrom columns are equipped with a twoposition media valve at the center of the bottom bed support. Introduction chromatography purification processes are a. Configured to satisfy the compatibility requirements for the entire range of axichrom chromatography columns from 300 to 1600 mm. When connected to an aktaprocess system, unicorn software is used as. Packing, unpacking, and maintenance of an axichrom column only takes 56% of the time required.
Packing smallscale axichrom columns this video from ge demonstrates packing and unpacking techniques for axichrom columns. Axichrom 100300 cytiva formerly ge healthcare life. Cmms software web based maintenance management software for doing equipment maintenance, facility maintenance and plant maintenance with features including work order tracking, preventive maintenance pm software and asset management all available through the internet using a web browser. Axichrom master cytiva formerly ge healthcare life. These two positions enable filling, packing, and unpacking without adjusting the assembled column. Axichrom ge healthcare 29046543 axichrom master 3001600. Axichrom columns are developed for use with a wide range of medium types. Ge axichrom 300 to 1600 mm chromatography columns by ge healthcare life sciences. Axichrom pressure gauge reference pressure gauge valve 3 water tank valve 1 valve 2 pump waste figure 5. The purpose of the column and the oven is to separate the injected sample into individual compounds as it travels through the column. Easy buffer management can also be integrated to the process. In process development, akta avant system brings efficiency to method development. View and download agilent technologies 5977b series operating manual online.
Choosing a backup generator plus 3 legal house connection options transfer switch and more duration. Process columns new and used process equipment labx. Page 57 agilent 5977b series msd operating manual intuvo 9000 gc column maintenance columns to replace an intuvo gc. Pivot or swingout column tubes are safer and easier to handle. The builtin computer with unicorn software allows standalone operation or integration into any plantwide control. Largescale columns for clinical to fullscale manufacturing. Preprogrammed automated methods for packing, unpacking, priming, and maintenance to simplify column handling and improve uptime. The swingout and swivel features on the axichrom column make maintenance convenient, secure, and effective, as they remove much of the extra work needed for disassembly fig 10. How to maintain smallscale axichrom chromatography columns cytiva formerly ge healthcare life sciences. The complete guide to microsoft wsus and configuration. Labx your marketplace to buy and sell process columns, pharma systems, scaleup columns, and more processing equipment. Axichrom 800 cytiva formerly ge healthcare life sciences. For special cleaning agents, please refer to chemical resistance information in.
Genentech roche designed and built it to meet network demand for a commercial launch facility, and placed it within an existing shell at the companys oceanside, ca, site. Axichrom automated columns cytiva formerly ge healthcare life. Operations and maintenance data management software, featuring antero cmms, operator10 wastewater, operator10 water and synexus pretreatment software, backed by worldclass technical support and services. Handling axichrom columns is easy using intelligent packing.
Axichrom master and unicorn wizards guide users through key process steps to save time in method creation, setup and maintenance. Resolute columns and packing systems maintain identical linear velocities and pressure profiles during the packing process, whether in a 400 mm pilotscale column or a 2000 mm processscale column. Overview cytiva formerly ge healthcare life sciences. How to maintain smallscale axichrom chromatography columns. Automated efficiency our akta chromatography systems support axichrom columns throughout scales. Axichrom 300 columns operating instructions 28956290 ad 5 maintenance and storage 5. Axichrom 300 to 1600 chromatography columns are controlled via axichrom master. Software program manager maintaining mass selective detectors msd less downtime for repairs. The axichrom column platform is a revolutionary concept in column chromatography that simplifies column handling at all scales from process development to fullscale production. Each column is designed for use with different compounds. Once the column is connected to the akta system, unicorn software controls the packing. Intelligent packing, a feature of axichrom master, enables the automated column packing and delivers verified methods and simplified workflows.
Construction materials in axichrom columns see page 8 are resistant to most chemical agents used in chromatography, including buffer solutions for adsorption, elution and washing, and to solutions effective in cleaning, sanitization and storage. Combining an axichrom column with a highflow medium can help drive towards lean biomanufacturing and operational excellence. The operator builds the packing method using the unicorn intelligent packing wizard. Packing, unpacking, and maintenance of an axichrom column only takes 56%. Ge healthcare launches axichrom columns laboratory talk. Capto s impact medium can be packed in axichrom columns to bed heights between 10 and 40 cm. Any use of unicorn software is subject to ge healthcare standard software. How to maintain largescale axichrom chromatography columns. This downstream train included new technologies, including the axichrom platform and column packing equipment to streamline column packing design. Data file 18111523 ad bioprocess columns bpg columns 100, 140, 200, 300 and 450 series imagination at work bpg columns are glass chromatography columns designed for industrial applications which demand high standards of hygiene. Different levels of automation are available through unicorn software and deltav. Page 6 agilent gc and gcms user manuals and tools dvd set agilent gcms software information and manuals memory stick see the agilent. The attached manual describes the xselect csh hplc columns and includes user recommendations. Streamlined columnpacking design for a new commercial.
Using the same packing methods reduces the effort required during preparation of sops for largescale manufacturing packing methods. Can be integrated via profibus into unicorn or a higher automation. Axichrom 300 columns user manual 28956289 ad 85 3 getting started 3. Axichrom 50, axichrom 100, and axichrom 400 sample. Compatible with the entire range of axichrom chromatography columns, from 300 to 1600 mm. The column dimension dictates which akta system to use. Column nut for gcms and two hole ferrules 0592121170 universal column. Packing, unpacking, and maintenance of an axichrom column only takes 56% of the time required for a traditional column.
Technology solutions simplifying chromatography operations. Maintenance schedule contamination mass spectral symptoms ion source. When you develop methods, follow the protocol for the adoption of analytical. Axichrom columns all column parts can be cleaned with the most commonly used agents, such as detergents, ethanol, weak acids, sodium hydroxide and high salt concentration. Find the best price on new or used process columns. Axichrom master cytiva formerly ge healthcare life sciences. Axichrom 300 to 1600 columns are controlled via axichrom master. Chromatographic performance and scalability from pilot.
Axichrom master controls the packing, unpacking and maintenance of large axichrom columns via a touchscreen interface. Axichrom columns introduce three key features intelligent packing, intuitive handling, and predictable scaleup. Packing of axichrom 50 to 200 columns is controlled by unicorn software, using an akta system. The following sql query can be run against the susdb database, to quickly determine the number of superseded updates. Smallscale columns for preclinical to pilot production. Lowpressure chromatography columns, in sizes 300 to 1600 mm internal diameter i. Axichrom columns are available with diameters from 50 to mm, allowing customers to choose the most suitable column size for their separation, from process development to fullscale production. Data file 28929041 am chromatography columns axichrom. Unlocking the potential for efficiency in downstream bioprocesses. Chromatography columns are used as the containers holding a bed of chromatography resin in purification of biotherapeutics and providing for even distribution of the material to be processed throughout this bed for best purification effect, capacity utilization, and volume throughput.
All columns have been designed to support the leanenabling approach to column operation and maintenance. The columns are constructed from component materials of the highest quality and withstand. Packing of capto s impact in bpg 100 and 300 and chromaflow 600 columns is also described. Automated chromatography column packing can save you both time and labor costs.
Intelligent packing, a feature of the unicorn control software, enables the automated column packing and delivers verified methods and simplified workflows. Set up the test with the axichrom column as in figure below. Table 1 lists the general chemical resistance of axichrom columns. Integrates via profibus into unicorn or a higher automation system, such as deltav distributed control system.
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